Examples: systemic administration of non-tissue irritating chemicals by routes that do not require surgery; food or water restriction of limited duration and magnitude (85% of pre-restriction body weight); limited periods of non-tissue damaging environmental stressors (e.g. brief periods of restraint [<4 hours], exposure to cold [20 degrees or less of temperature to which acclimated]); ear tagging; injections; blood sampling; tube feeding; behavioral experiments without significant restraint; anesthesia studies; live capture for weighing, measuring, etc.; behavioral experiments (i.e.: tail flick test in rats); euthanasia for museum collection; post mortem tissue collection following an approved, non-mechanical method of euthanasia; euthanasia via decapitation of anesthetized adult rodents.

Note: Any new procedure thought to fulfill Class I requirements, may, as the research proceeds, be found to result in pain and/or distress which could not have been reasonably predicted, and for which the use of analgesics, anesthetics or tranquilizers is prohibited (See Class III below). In this instance, the use of this procedure for any animal which avoids the use of anesthetics, analgesics, or tranquilizers, will require submission of an Animal Use Statement revision requesting approval of this procedure by the IACUC under Class III.

Examples: Terminal exsanguination, biopsies, blood vessel exposure, catheter implants, surgical procedures, relatively small burns (brands), nephrotomy, brain exposure, ovariectomy.

Examples: Induction of certain disease states; radiation treatment to induce radiation sickness; purposeful/non-purposeful induction of infection; stress and/or shock treatments; certain toxicity experiments which lead to death and/or sickness; prolonged water/food restriction [use of the word ÒrestrictedÓ in all instances may be qualified by species involved]; prolonged exposure to low/high temperature, or restraint; experiments that allow manifestation of convulsions, severe diarrhea and/or vomiting (e.g. withdrawal following highdose acute or repeated addictive drug administration); monoclonal antibody studies which involve ascites induction; polyclonal antibody studies which do not follow approved IACUC protocols and which may be expected to be painful; polyclonal antibody studies which lead to prolonged organ dysfunction; infliction of 2nd/3rd-degree burns or severe trauma; procedures which produce psychotic-like behavior; certain euthanasia methods [may be species dependent].